Calculate precise lab dilution factors instantly. Simplify experiments, avoid errors, and gain confidence with our easy-to-use online dilution calculator.
You’ve got this—just follow these quick steps to get your result:
The dilution factor (DF) quantifies how much a solution has been diluted from its original concentration. It is a critical metric in laboratory sciences for accuracy and reproducibility.
Dilution Factor (DF) = Final Volume (Vf) / Initial Volume (Vi)To determine the new concentration after dilution:
Final Concentration (Cf) = Initial Concentration (Ci) / DFA lab technician dilutes 50 µL of blood to 1 mL:
DF = 1000 µL / 50 µL = 20This results in a 20-fold dilution before biochemical testing.
A biologist dilutes a 250 ng/µL DNA sample to 5 ng/µL:
DF = 250 / 5 = 50They dilute 2 µL to a total volume of 100 µL to achieve this.
Total DF = DF₁ × DF₂ × DF₃ ... × DFnStart calculating now and take the guesswork out of your next dilution step!
| Initial Volume (µL) | Final Volume (mL) | Dilution Factor |
|---|---|---|
| 100 | 10 | 100 |
| 50 | 5 | 100 |
| 10 | 1 | 100 |
| 25 | 2.5 | 100 |
| 1 | 0.1 | 100 |
| 0 | 1 | Invalid |
| 100 | 100 | 1 |
| 500 | 250 | 0.5 |